The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family (2024)

Parker, K. E. R., Fairweather, S. J., Rajendran, E., Blume, M., McConville, M. J., Bröer, S., Kirk, K., & van Dooren, G. G. (2019). The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family. PLoS Pathogens, 15(2), Article e1007577. https://doi.org/10.1371/journal.ppat.1007577

Parker, Kathryn E.R. ; Fairweather, Stephen J. ; Rajendran, Esther et al. / The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family. In: PLoS Pathogens. 2019 ; Vol. 15, No. 2.

@article{08ef70bbd82c45938f5cffb620fbc581,

title = "The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family",

abstract = "Apicomplexan parasites are auxotrophic for a range of amino acids which must be salvaged from their host cells, either through direct uptake or degradation of host proteins. Here, we describe a family of plasma membrane-localized amino acid transporters, termed the Apicomplexan Amino acid Transporters (ApiATs), that are ubiquitous in apicomplexan parasites. Functional characterization of the ApiATs of Toxoplasma gondii indicate that several of these transporters are important for intracellular growth of the tachyzoite stage of the parasite, which is responsible for acute infections. We demonstrate that the ApiAT protein TgApiAT5-3 is an exchanger for aromatic and large neutral amino acids, with particular importance for L-tyrosine scavenging and amino acid homeostasis, and that TgApiAT5-3 is critical for parasite virulence. Our data indicate that T. gondii expresses additional proteins involved in the uptake of aromatic amino acids, and we present a model for the uptake and homeostasis of these amino acids. Our findings identify a family of amino acid transporters in apicomplexans, and highlight the importance of amino acid scavenging for the biology of this important phylum of intracellular parasites.",

author = "Parker, {Kathryn E.R.} and Fairweather, {Stephen J.} and Esther Rajendran and Martin Blume and McConville, {Malcolm J.} and Stefan Br{\"o}er and Kiaran Kirk and {van Dooren}, {Giel G.}",

note = "Publisher Copyright: {\textcopyright} 2019 Parker et al. http://creativecommons.org/licenses/by/4.0/.",

year = "2019",

month = feb,

doi = "10.1371/journal.ppat.1007577",

language = "English",

volume = "15",

journal = "PLoS Pathogens",

issn = "1553-7366",

publisher = "Public Library of Science",

number = "2",

}

Parker, KER, Fairweather, SJ, Rajendran, E, Blume, M, McConville, MJ, Bröer, S, Kirk, K & van Dooren, GG 2019, 'The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family', PLoS Pathogens, vol. 15, no. 2, e1007577. https://doi.org/10.1371/journal.ppat.1007577

The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family. / Parker, Kathryn E.R.; Fairweather, Stephen J.; Rajendran, Esther et al.
In: PLoS Pathogens, Vol. 15, No. 2, e1007577, 02.2019.

Research output: Contribution to journal › Article › peer-review

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T1 - The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family

AU - Parker, Kathryn E.R.

AU - Fairweather, Stephen J.

AU - Rajendran, Esther

AU - Blume, Martin

AU - McConville, Malcolm J.

AU - Bröer, Stefan

AU - Kirk, Kiaran

AU - van Dooren, Giel G.

N1 - Publisher Copyright:© 2019 Parker et al. http://creativecommons.org/licenses/by/4.0/.

PY - 2019/2

Y1 - 2019/2

N2 - Apicomplexan parasites are auxotrophic for a range of amino acids which must be salvaged from their host cells, either through direct uptake or degradation of host proteins. Here, we describe a family of plasma membrane-localized amino acid transporters, termed the Apicomplexan Amino acid Transporters (ApiATs), that are ubiquitous in apicomplexan parasites. Functional characterization of the ApiATs of Toxoplasma gondii indicate that several of these transporters are important for intracellular growth of the tachyzoite stage of the parasite, which is responsible for acute infections. We demonstrate that the ApiAT protein TgApiAT5-3 is an exchanger for aromatic and large neutral amino acids, with particular importance for L-tyrosine scavenging and amino acid homeostasis, and that TgApiAT5-3 is critical for parasite virulence. Our data indicate that T. gondii expresses additional proteins involved in the uptake of aromatic amino acids, and we present a model for the uptake and homeostasis of these amino acids. Our findings identify a family of amino acid transporters in apicomplexans, and highlight the importance of amino acid scavenging for the biology of this important phylum of intracellular parasites.

AB - Apicomplexan parasites are auxotrophic for a range of amino acids which must be salvaged from their host cells, either through direct uptake or degradation of host proteins. Here, we describe a family of plasma membrane-localized amino acid transporters, termed the Apicomplexan Amino acid Transporters (ApiATs), that are ubiquitous in apicomplexan parasites. Functional characterization of the ApiATs of Toxoplasma gondii indicate that several of these transporters are important for intracellular growth of the tachyzoite stage of the parasite, which is responsible for acute infections. We demonstrate that the ApiAT protein TgApiAT5-3 is an exchanger for aromatic and large neutral amino acids, with particular importance for L-tyrosine scavenging and amino acid homeostasis, and that TgApiAT5-3 is critical for parasite virulence. Our data indicate that T. gondii expresses additional proteins involved in the uptake of aromatic amino acids, and we present a model for the uptake and homeostasis of these amino acids. Our findings identify a family of amino acid transporters in apicomplexans, and highlight the importance of amino acid scavenging for the biology of this important phylum of intracellular parasites.

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U2 - 10.1371/journal.ppat.1007577

DO - 10.1371/journal.ppat.1007577

M3 - Article

SN - 1553-7366

VL - 15

JO - PLoS Pathogens

JF - PLoS Pathogens

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Parker KER, Fairweather SJ, Rajendran E, Blume M, McConville MJ, Bröer S et al. The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family. PLoS Pathogens. 2019 Feb;15(2):e1007577. doi: 10.1371/journal.ppat.1007577